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文献和实验Applications of Ultra-high-Throughput Sequencing
the molecular basis of biological questions to be directly addressed, to an even greater degree, ultra-high-throughput DNA sequencing is poised to dramatically change the nature of biological research. New sequencing technologies have opened the door for novel
have rendered better results in terms of yield and cell type representation, while minimizing cell aggregation (3 , 9 ). Nevertheless, they are time-consuming and involve a lot of handling. Here, we present a very simple and efficient protocol
96-well RNA In Situ Hybridization Protocol
The RNA in situ procedure described below is based on the protocol developed by Tautz and Pfeifle (Chromosoma 98 (1989), p81), but adapted to allow the screening of 96 RNA probes on whole-mount Drosophila embryos at the same time.Jasprien
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