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文献和实验Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
. () View Image Figure 27.3.7 Protein microarray layout and mask fitting. (A ) Scanned image from a protein microarray slide (Cy3—green; Cy
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
). II. Procedure A. Cell treatment and collection Farm L929 cells on 150mm plates (10 plates yeilds approx. 550ug of protien). Treat cells with latex beads (Polybead polystyrene microspheres 4.55 x 10 beads/ml
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