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文献和实验with this for 30min to 1 hr. 5. Primary incubation. Make a solution of 1℃ antibody in blotto (or BSA/TBS/Tween). Need only ~5 mls or less for a modest size blot (14X8 cm). Antibody concentration should be optimized; a general guidline is ~1:2000 dilution for a red
Hints and precautions for the care, feeding and breeding of Neurospora
. For most manipulations and observations (ascospore isolations; inspection of spores, perithecia, etc.), an excellent general-purpose microscope is the Stereo Zoom 7 with base, Bausch & Lomb Cat. No. BVIO70 with 10X oculars (1984 price $1417). This provides 10X-70X
Hints and precautions for the care, feeding and breeding of Neurospora
general-purpose microscope is the Stereo Zoom 7 with base, Bausch & Lomb Cat. No. BVIO70 with 10X oculars (1984 price $1417). This provides 10X-70X magnification. Illumination from above is best for isolating ascospores, with a white plate
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