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文献和实验complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl
Assessment of Circadian and Light‐Entrainable Parameters in Mice Using Wheel‐Running Activity
) Data collection computer and software to record wheel running activity (e.g., ClockLab, http://www.actimetrics.com/ClockLab/; VitalView, http://www.minimitter.com/vitalview_software.cfm; The Chronobiology Kit, http://www.query.com
) Nuclear lysis buffer Oligonucleotide linkers (optional; see Step 18) PBS (phosphate-buffered saline [pH 7.4]) PCR product purification kit (e.g., QiaQuick PCR purification kit, QIAGEN) PCR reagents (e.g., Taq polymerase
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