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文献和实验Size‐Exclusion Chromatography with On‐Line Light Scattering
the use of size?exclusion chromatography with on?line light scattering, UV absorbance, and refractive index detectors (SEC?LS/UV/RI) to determine: (a) the molecular weight of simple proteins containing no carbohydrates, (b) the molecular weight
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
Replication timing by density transfer
Replication timing by density transfer M. K. Raghuraman 1. Grow cells at least 7 generations in 0.1% (w/v) [13
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