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文献和实验Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
previously. Do not use an mouse that appears lighter than 25g, as underweight mice tend to re-absorb the embryos as they are not physically ready to support a pregnancy. Overweight mice can make surgery difficult by the absorption of anaesthetic into the fat
as a control. Use a glass rod to mix thoroughly the contents of both tubes and then leave them in a boiling tube rack in a water bath at 35°C for at least 30 minutes. Take two similar sized funnels (funnel size about 100 cm
to one of the tubes and 25 cm3 of water to the other, to act as a control. Use a glass rod to mix thoroughly the contents of both tubes and then leave them in a boiling tube rack in a water bath at 35°C for at least 30 minutes.
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