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文献和实验Denaturing Discontinuous Polyacrylamide Gel Electrophoresis (SDS-PAGE)
TEMED 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 These recipes produce 30 ml of separating gel, adequate for casting four minigels of dimensions 0.75 mm x 7.3 cm x 8.3 cm. Units of all numbers in the table are milliliters
The compilation of standard balanced salt solutions with their references found in the following table can be helpful in selecting an appropriate dissociation solution. Standard Solution Table Composition of Selected
-HCl pH 9, 15 mM MgCl, 0.1 gelatin w/v, 1 Triton X-100), 1 ul of 50 uM primer, 1 ul of 1 mM dNTP''s and 1 ul DNA solution. Tubes were boiled for 2.5 min. Tubes were returned to ice, then 1 unit of Taq polymerase (Promega) was added. Tubes were spun
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