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文献和实验Sleep and EEG Phenotyping in Mice
/) Stainless steel miniature screws (e.g., Precision Screws and Parts, 303 SS, diameter 1.1 mm, Morris Co.; http://www.morris01550.com/; gold plated by a local gilding/galvanic workshop; the gold plating is not essential to the protocol
Identification and Analysis of Multiprotein Complexes Through Chemical Crosslinking
) Extract buffer (see recipe ) 0.5‐mm glass beads, (e.g., Scientific Instruments), prechilled to −20°C
Strategies to Optimize Protein Expression in E. coli
Bao, W.J., Gao, Y.G., Chang, Y.G., Zhang, T.Y., Lin, X.J., Yan, X.Z., and Hu, H.Y. 2006. Highly efficient expression and purification system of small‐size protein domains in Escherichia coli for biochemical characterization. Protein Expr
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