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文献和实验products by utilizing four primers instead of the usual two in a PCR reaction. This protocol is particularly useful when cloning large numbers of genes (universal att B adapter primers can be synthesized once and used hundreds of time), or when additional
AFLP: not only for fingerprinting, but for positional cloning
to another but is of a single, defined sequence within a given primer. The selective nature of AFLP-PCR is based on the 3' extensions on the oligonucleotide primers. Since these extensions are not homologous to adapter sequence, only plant DNA fragments complementary
AFLP: not only for fingerprinting, but for positional cloning
. Since these extensions are not homologous to adapter sequence, only plant DNA fragments complementary to the extensions will be amplified due to the inability of Taq DNA polymerase, unlike some other DNA polymerases, to extend DNAs if mismatches occur at the 3'-end
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