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上海希言科学仪器有限公司
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文献和实验: cold9. chloroform/butanol: 4:lThe following is protocol for 1.6 g of needle or phloem tissue, which is a suitable volume for one 50-ml orange cap tube (Corning, conical) that you will need for processing. Scale up by nX the following protocol:Chill
Screening Colonies by Hybridization with Radiolabeled Probe
bath for 5 minutes, then centrifuge briefly at room temperature to collect condensed liquid from cap of tube. Add the following reagents to the reaction tube: 10 µl of 5 x primer buffer (either 5 x *dATP or 5 x *dCTP buffer
Synthesis of Oligodeoxynucleotides with 5′‐Caps Binding RNA Targets
of a Molecular Cap Building Block Alternate Protocol 1: Synthesis of 2′‐Oligodeoxyribonucleotides with 5′‐Cap Build Up on Solid Support Support Protocol 1: Synthesis of N‐Fmoc‐L‐Prolinol Linker Phosphoramidite
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