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文献和实验Protein Expression and Purification of MBP-T antigen (131-259)
thrombin (200U for 6 L prep.)and dialyze sample against buffer CO/N in cold room.Make sure the cleavage is complete.If it is not complete let the reaction go at room temperature with another addition of 100μl of thrombin. xvi.Pass the sample over pre
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
amounts of lysate(usually 0.1–10 μL in a total volume of 100 μL of water) to1 mL of Coomassie (Bradford) reagent, vortex, and incubateat room temperature for 10 min. Vortex the solutions again,then measure the absorbance using a spectrophotometer atl max
membrane proteins (see protocol 8 and ) Native standard proteins (e.g., in the range of 100‐ to 1000‐kDa) Sample buffer (see reciperecipes ) Anode
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