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文献和实验Sample preparation (analytical gels)
. ELC (erythroleukemia cell line): A monolayer culture of human ELC was grown, rinsed trypsinized and washed as explained in the HEPG2 sample preparation. A pellet of 0.8 x 106 cells were mixed and solubilized with 60 μl of a solution containing urea
Sample preparation (preparative gels)
. Five hundred µl containing 5 x 106 kidney cells was used for in-gel sample rehydration. 2 and 3 ]. LIVER Protocole 2: Ten mg of a human liver frozen biopsy was lyophillized for 48 h. It was then crushed in a mortar containing liquid nitrogen
Sample preparation (analytical gels)
,rinsed trypsinized and washed as explained in the HEPG2 sample preparation.A pellet of 0.8 x106 cells were mixed and solubilized with 60 µl of a solution containing urea (8 M),CHAPS (4% w/v),Tris (40 mM),DTE (65 mM)and a trace of bromophenol blue
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