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文献和实验by aspiration.4) Add 1.0ml new Lysis buffer. Vortex (15-30s) to break up pellet.6) Centrifuge for 15s at 12,000 rpm. Remove red supernatant by aspiration.** at this stage nuclear pellets may be stored long term by adding 100μl TEN buffer and freezing at 20
. SPRi experiment The spotted biochip was inserted into the SPRi-PlexII™ instrument from HORIBA Scientific. The system is equ-ipped with a 200 μL sample loop,a continuous flow pump and an in-line degasser. The flow rate
Protein Expression and Purification of MBP-T antigen (131-259)
thrombin (200U for 6 L prep.)and dialyze sample against buffer CO/N in cold room.Make sure the cleavage is complete.If it is not complete let the reaction go at room temperature with another addition of 100μl of thrombin. xvi.Pass the sample over pre
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