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上海希言科学仪器有限公司
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文献和实验oil. PCR according to taste (not inhibited by presence of a little bromophenol blue or of 50ng/ml ethidium bromide). 8. At end of PCR: if you allow the tubes to cool down the reaction mix will set: 2%-odd agarose diluted 1/5 sets quite
Preparation of total RNA from whole blood collected in PAXgene tubes
into a 1.5 ml or 2 ml microcentrifuge tube (not supplied). Add 300 µl Buffer BR2 and 40 µl Proteinase K. Mix by vortexing, and incubate for 10 min at 55℃ using a shakerincubator, heating block, or water bath.If a heating block or water bath is used, vortex
RNA AND PROTEIN EXTRACTION FROM THE SAME SAMPLE
Tris-HCl pH 8; 1% SDS, 1% deoxycholate-sodium, 20 mM EDTA (2% PVP 40,000-optional when using tissues with high conconcentration of phenols) 2. Phenol: Add to 200 mL liquified phenol 200 mg of antioxidant
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