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上海希言科学仪器有限公司
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文献和实验and rinse the cells with 10ml of warmed PBS, aspirating the PBS off. Add 2ml of warmed 0.05% trypsin/EDTA, covering the PMEF layer. Place the flask on the warming tray for 2 minutes, then gently tap the sides of the flask to dislodge the cells.
Thawing Cells - Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. - Add the 1ml
ES Cell Culture and Manipulation
/streptomycin 1X Non-essential amino acids 1X ribonucleosides 1/100 volume ß-ME stock (stock is 7µl ß-ME in 10ml DMEM) 1:1000 dilution of LIF (add 500 µl 720LIFD cell conditioned media to 500ml media) Thawing frozen vials of ES cells
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