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文献和实验recirculation during the run, so put a stir bar in each buffer tank, set up a peristaltic pump to recirculate buffer between the two tanks. Put a stir plate under each buffer tank. 4. Chill the RNA tubes on ice, spin them down briefly in the microfuge. Add 4 µl
the stir bars and peristaltic pump; this allows the RNA to run into the gel first so that the buffer circulation won't disturb it in the wells. It takes 2.5 hours to run the gel about 11 cm; the gel and buffer will become warm during the run.
after turning on the power before starting the stir bars and peristaltic pump; this allows the RNA to run into the gel first so that the buffer circulation won't disturb it in the wells. It takes 2.5 hours to run the gel about 11 cm; the gel and buffer
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