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上海希言科学仪器有限公司
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文献和实验, swirl to mix, and incubate in an ice-water bath for 1-2 hours. 8a. Collect the PEG-precipitated DNA by centrifugation in 250 ml bottles at 7000 rpm for 20 minutes at 4degC in the RC5-B using the GSA rotor. 9a. Dissolve the pellets in a combined
Methods for DNA isolation(DNA分离方法)
by centrifugation at 7000 rpm for 20 minutes in 500 ml bottles in the RC5-B using the GS3 rotor.Resuspend the cell pellets in old media and transfer to two bottles,centrifuge as before,and decant the media.The cell pellets can be frozen at -70degC at this point
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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