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文献和实验Purification of recombinant sBRF M166L
Tris 300mM NaCl 0.87 g NaCl 8.0M Urea 24 g Urea 100mM Imidazole 0.33 g Imidazole H2O to 50 ml finalRenaturation of BRF protein Thaw Ni-NTA purified samples.Dilute the sample to a concentration of 0.25 to 0.5mg/ml in the elution buffer
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
pellet. H. Pre-warm 6-well plates with 2 ml of complete medium at 37 °C. I. Add 2 μg of Target ID Library (not to exceed 10 μl) per 0.5 ml tube for each transfection. J. Resuspend cells in the 15
) at the final concentration of 1 tablet/50ml): PBS Buffer A: 10 mM Hepes, pH 7.9, 10mM KCl, 1.5mM MgCl2, 0.5mM DTT S1 solution : 0.25 M Sucrose, 10 mM MgCl2 S2 solution: 0.35 M Sucrose, 0.5 mM MgCl2 S3 solution: 0.88 M Sucrose, 0.5 mM MgCl2
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