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文献和实验and process following the protocol described in the PureLink™ Micro-to-Midi™ RNA Purification System manual available there . Determine the quality and quantity of the purified RNA using UV absorbance at 260 nm or Quant-iT™ RNA Assay Kit (cat
Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
, i.e. to immediately transform the fatty acid glycerol esters into fatty acid methyl esters. This was achieved in the transmethylation reaction with BF3 and methanol. The mobile phase in GLC is an inert gas (nitrogen); fatty acid methyl esters
Immuno-Laser Capture Microdissection
Staining kit (DAKO Corp.), a three-step streptavidin-biotin technique with prediluted mono- or polyclonal (rabbit) primary antibodies optimized for very short staining times. Diaminobenzidine, DAB Hematoxylin solution, Mayer's (Sigma
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