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文献和实验of an inert carrier, such as 20 micrograms glycogen, helps visualize the DNA pellet. Wash pellets with 70% ethanol and air dry. 15, Resuspend pellets in an appropriate buffer for PCR or slot-blot reactions. PCR or slot-blot conditions
HPLC-Chip/MS Technology in Proteomic Profiling
preconcentration and separation with fluidic connection made automatically in a leak-tight fashion. Minimum precolumn and postcolumn peak dispersion and uncompromised ease of use result in compounds eluting in bands of only a few nanoliters. The chip is fabricated
Promoter analysis by saturation mutagenesis
can be used directly for cloning as a blunt-ended insert using the Sureclone ligation kit (Amersham Pharmacia Biotech) or digested with restriction enzymes for directional cloning (if unique restriction sites are incorporated at the 5' ends of the primers
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