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文献和实验Two dimensional nano-high-performance liquid chromatography (nanoHPLC) coupled directly to a high-resolution tandem mass spectrometer (2D-nLC-MS/MS) is an excellent method for analyzing very complex peptide mixtures, especially
and their structural characterization in situ using tandem ESI and MALDI mass spectrometry. Detailed protocols are provided that describe the application of nano-LC-ESI-MS/MS with alternative fragmentation modes (collision-induced dissociation vs. electron-transfer
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
, several methods have been developed to analyze protein?protein interactions under native conditions. One of them, tandem affinity purification (TAP), combines two affinity?purification steps to allow isolation of high?purity protein complexes. This unit presents
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