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文献和实验Combination of 2DE and LC for Plant Proteomics Analysis
these results in consideration, we propose that 2DE and liquid chromatography are complementary methods to analyze complex protein extracts and can be used in parallel to acquire a wider perspective and a better understanding of the embryo proteome.
RASL‐seq for Massively Parallel and Quantitative Analysis of Gene Expression
10 mM dNTPs NucleoSpin Extract II kit for PCR purification (Clontech, cat. no. 740609.50)
revealed the presence of large amounts of chemical–protein adducts in both the OSOM and urine. By aligning the Western blot film with a parallel gel stained for protein, we can isolate the adducted proteins for LC-MS/MS analysis. Subsequent database
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