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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
antibody (mAb) conjugated with peroxidase (anti-DNA-POD) was used. This anti-DNA-POD mAb binds to single- and double-stranded, low molecular weight DNA fragments (mono- and oligonucleosomes), showing the internucleosomal degradation of genomic DNA occuring
Immuno-Laser Capture Microdissection
Staining kit (DAKO Corp.), a three-step streptavidin-biotin technique with prediluted mono- or polyclonal (rabbit) primary antibodies optimized for very short staining times. Diaminobenzidine, DAB Hematoxylin solution, Mayer's (Sigma
) and that provided by CLONTECH in the Advantage-GC cDNA PCR kit is in fact Betaine which is available at a fraction of the cost as a 5M solution from Sigma-Aldrich (cat. # B 0300), but be sure to use Betaine or Betaine (mono)hydrate and not Betaine HCl
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