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文献和实验SSR GEL and Silver Staining Protocol
Service Research 5985 Highway K Rhinelander, WI 54501 USA Phone: 715-362-1121 Fax: 715-362-1166 e-mail: pmarquar@newnorth.net I. EQUIPMENT: DNA sequencing unit (35 x 45 cm) & 2000V power supply Clamps Lg. plastic trays (4), about 43 x 50 x
activity 3000 Ci/mmol; Amersham Pharmacia Biotech) Drosophila Schneider SL2 tissue culture cells or other tissue or cell line for analysis, with appropriate growth facilities and equipment Ethanol (100% and 70%) Ethidium bromide (10 mg
ES Cell Culture and Manipulation
of bubbles down) and check individually under the 4X microscope to be sure no clumps are left. Transfer each disaggregated clone to a numbered 24-well feeder (the 2ml media will neutralize the trypsin). Notes: 1.) Change yellow tips for each clone
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