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文献和实验Injection of C. elegans Prepare injection pads line up three 22x50 mm coverslips place drop of molten 2% agarose on each quickly place slide on top of the agarose and wait 20 seconds (see figure
Determination of Polyamines as Their Benzoylated Derivatives by HPLC
). The advantages of this technique are its relative simplicity and the need for only very basic HPLC equipment—pump, column, injection valve, and UV-detector. The main disadvantage, as noted by Seiler ( 13 , 14 ), is that benzoyl chloride, in addition to undergoing
including the point of sample introduction. In an on-column injection, the needle must enter the column inside the oven. The injector used has a butterfly valve, which is opened as the turret of the autosampler rotates over the injector, . The sample is
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