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文献和实验SequalPrep Long PCR Kit with dNTPs
, 5 U/μl 0.36 μl 1.8 U DNase-free water to 18 μl — 3. Pipette 18
C-kit Expression Identifies Cardiac Precursor Cells in Neonatal Mice
receptor c-kit. Utilizing a bacterial artificial chromosome (BAC) transgenic mouse model in which EGFP expression is placed under control of the c-kit promoter (c-kitBAC -EGFP), work from our laboratory indicates that c-kit expression identifies
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
medium and discard. To the bacterial pellet add 250 u Solution I/RNase A. Resuspend cells completely by vortexing or pipetting up and down. Complete resuspension of cell pellet is vital for obtaining good yields. 4. Add 250 u Solution II and mix
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