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文献和实验. 2. Also collect T-cells as a flow cytometry control. 3. Collect low density cells by equilibrium centrifugation over Ficoll-Hypaque (d=1.077 g/mL). Alternatively, RBC may be lysed. See blood flow cytometry protocol for details. 4. Wash cells in PBS/FBS
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
实验试剂 SDS based extraction solution 实验步骤 1. Harvest cells. 1) Trypsinize and collect cells, aspirate medium, and wash cells with PBS twice. 2) Determine the number of cells.
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