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文献和实验Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
on a glass slide ~7-10 mm apart and cover with a 18x18 or 22x22 mm coverslip. This results in a ~12-15 µl flow cell. Solutions are pipeted on one side and sucked out the other side by capillary action using Whatman #1 filter paper or a Kimwipe. Washes in flow
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
of the method for concurrent flow cytometric measurement of two cell-surface antigens and DNA-RNA content. Human peripheral blood mononuclear cells were cultured for 72 h, either in medium alone (A-D ) or in medium containing 100 ng/mL of soluble CD28.2
A general protocol for staining cell for cytometry analysis
to the cell pellet. Gently mix the cells and incubate at RT for 15 min in the dark. 6. Add 400 µl of 1× Binding Buffer to each tube. Analyze by flow cytometry as soon as possible (within 1 hr).
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