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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
, cell-type-specific mapping of all the chromatin interactions mediated by specific proteins. The 6C assay advances our understanding of the three-dimensional aspects of the higher-order folding of chromatin and provides an important tool to examine
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
from the kit to the column. 17. Let stand for 1 minute. 18. Centrifuge at 2880 x g for 5 minutes. 19. Add another 1 ml of water to the column. 20. Let stand for 1 minute. 21. Centrifuge at 2880 x g for 10 minutes. 22. Aliquot
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