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文献和实验Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture
and the concentration of metabolic waste products play a major role in the performance of the cell culture. In order to monitor the concentrations of these substances and to evaluate the state of the culture, some different methods have to be applied. There is a broad
ii. Add 0.3 or 0.6 mL Lysis Buffer with 2-mercaptoethanol to the sample (see table above for volume). iii. Vortex until the cell pellet is dispersed and the cells appear lysed
LIVE/DEAD® Violet Viability/Vitality Kit
protocol has been optimized using Jurkat cells (human T-cell leukemia line) at a concentration of 1 × 106 cells/mL. Use of other cell types or other cell concentrations may require optimization of staining. If another staining reaction is to be performed
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