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文献和实验DNeasy 96植物技术:使用搅拌磨MM 300从新鲜植物叶片中分离DNA
from each rack of collection microtubes. To collect any solution from the caps, centrifuge the collection microtubes. Allow the centrifuge to reach 3000 rpm, and then stop the centrifuge. Do not prolong this step (将插入微型收集管架的两个适配微孔板取出,并除去适配微孔板。收集盖子里的溶液,将收集微型管进行离心
µCi/µl, specific activity 3000 Ci/mmol) (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)dNTPs (stock solutions at 25 mM for each dNTP)DNA loading buffer (6X)30% (v/v) glycerol0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanol FFStore
Using MicroRNAs to Enhance the Generation of Induced Pluripotent Stem Cells
cell suspension and diluted to a final concentration of 1 × 105 ∼1.5 × 105 cells/ml. Cells were then seeded in 20‐µl drops onto the cover of petri dishes to reach 2000∼3000 cells/drop. Embryoid bodies (mEBs) were formed by inverted culture of the cells
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