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文献和实验CellTrace CFSE Cell Proliferation Kit
Materials Needed but Not Included: Cells of interest as a single-cell suspension PBS/0.1% BSA 5 mM stock solution of CFSE probe (see Reagent Preparation) Culture media for cells of interest Flow cytometer with 488
Endothelial Cell Migration Under Flow
and migration within the direction of flow. Failure to adapt to shear stress results in endothelial damage contributing to generation of atherosclerotic plaques or abnormal vessel repair. This chapter describes methods of generating laminar flow in vitro
Analysis of Endothelial Cell Migration Under Flow
migration under well-defined flow conditions. Time-lapse microscopy and cell tracing analysis generated quantitative information on EC migration under flow, and this assay was used to dissect the signaling events involved in shear stress-induced EC migration
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