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文献和实验Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
of plasmids containing the viral EBV origin of replication. This protocol describes the instructions for the growth, maintenance and transfection of suspension adapted 293-EBNA cell line using the PEI expression system. Cells can be purchased from ATCC
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
of plasmids containing the viral EBV origin of replication. This protocol describes the instructions for the growth, maintenance and transfection of suspension adapted 293-EBNA cell line using the PEI expression system. Cells can be purchased from ATCC
Intracellular Calcium Assays in Dissociated Primary Cortical Neurons
In this chapter, the basic tools and methods to isolate neurons from the embryonic rat cortex are provided. We outline the isolation of fresh neuronal cells and their storage, post-thaw maintenance, and the application of techniques
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