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文献和实验for 5 min. Transfer 600 μL the supernatant into a new 2 ml tube and add 200 μL Buffer SP2. Mix the sample throughly by vortexing. 5. Incubate on ice for 5 minutes. Centrifuge at 13,000 x g in a microcentrifuge for 5 minutes. 6. Carefully
This protocol is based on enrichment from 1 x 107 leucocytes (MNC). It is scalable from 1 x 107 –2 x 109 cells. Keep the cells and buffers cold (2–8°C) during the whole process. 1) Transfer 100 μl (1 x 107 ) leucocytes in Buffer
Dynabeads® Co-Immunoprecipitation Kit
Dynabeads® Co-Immunoprecipitation Kit Magnet: e.g. DynaMag™-2 Mixer allowing rotation or tilting of tubes. Antibodies of your choice. 实验步骤 Day 1 1. Weigh out the appropriate
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