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上海希言科学仪器有限公司
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文献和实验case, the antibody should be added in 1:100 to 1:500 final dilution. • For Hsp70, 3A3 and C92 did work, 4G4 and 5A5 did not. 3A3 may be used 1 μl/ sample. • HSE probe must have high-specific activity (>10,000 cpm/fmol) and be double-stranded
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
it. Add 100 µl of saturated NaCl (5M) and 600 µl of chloroform to eppendorf tube and mix on a rotating blood mixer at room temperature then spin it for 2 minutes at 7000 rpm. Transfer 400 µl of supernatant to a new 1.5 ml tube. Add 800 µl
DNeasy 96植物技术:使用搅拌磨MM 300从新鲜植物叶片中分离DNA
solution into each collection microtube. Seal the microtubes with the provided caps (按照下表将AP1 缓冲液、 RNase A 及试剂DX 混合配成裂解工作液。吸取裂解工作液在每个 微型收集管 加入400 µl 。用提供的盖子将 微型收集管封好 )。It is important to prepare fresh working lysis solution. To allow thorough mixing
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