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Viper Parallel Setup Kit, RS System
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A/B/C Random Amplification Protocol
However part B should be able to handle much larger quantities of sequence tagged substrate than is produced in the Part A reaction. For two color arrays an equal amount of control DNA should also be amplified in parallel to the experimental DNA.
containing chloramphenicol (Cm, 20 μg/ml)/streptomycin (Stp, 75 μg/ml) and purified using the Large construct kit (Qiagen, Valencia, CA, USA). PCR PCR reactions using oligonucleotides carrying HAs were performed using
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Viper Parallel Setup Kit, RS System
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