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上海希言科学仪器有限公司
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文献和实验Preparation of Poly A+ RNA and Northern Analysis
at 56°C and pour denaturing gel (mid-size gel: to 0.8 gm of agarose, add 78.4 ddH2O and 5 ml of 20 x E buffer; heat to dissolve; let cool to not too hot to touch, then quickly add 16.6 ml of 37% formaldehyde, swirl to thoroughly mix and pour). Spin
cDNA Library Screening Protocol - ZAPII
at 30°C. 3) Spin for 10 min at 2000 rpm in a sterile 50 ml conical using Beckman GS 6R centrifuge. Carefully decant media and gently resuspend pellet in 15 ml of 10 mM MgSO4 (don't vortex). Dilute cells to OD600 = 0.5 with 10 mM MgSO
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
dihydrochloride Kodak #X8748 DNA PAGE % gel 3.5 5.0 8.0 12.0 20.0 30% acryl stock 11.6 16.6 26.6 40.0 66
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