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上海希言科学仪器有限公司
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文献和实验Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
Reagents: NGS-Normal Goat Serum PBST-0.5% Tween-20/CMF-PBS CMF-PBS 1°Ab-mouse MF20 (anti-myosin monoclonal) 1°Ab-rabbit myoD (anti-myoD polyclonal) 2°Ab-goat anti-mouse IgG-FITC 2°Ab-goat anti-rabbit IgG-FITC
Western Blot of TBP from TBP-GST bacteria
lysate in 9uL 2xLSB on gel (load all 10uL in one lane) 11. Block in TBST w/ 5% milk ON @ 4℃ 12. Primary Ab- use TBP (58C9) 1:400 in TBST w/ 5% milk for 2 hours 13. Secondary Ab- use mHRP 1:25000 in TBST w/ 5% milk for 2 hours 14. ECL
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