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文献和实验Practical Tips for Optimal Northern Analysis
though a wick of blotting paper (such as Whatman 3MM), the gel, the membrane and into the dry towels. The capillary transfer set up can be upward or downward (Figure 1). Regardless of the method chosen there are hints that can optimize any capillary transfer
Southern Blot Analysis(from Baker lab, university of Florida
mL 0.5M NaH2PO4/ 2 liter). 4.Cut Hybond N+ to the size of the gel and wet with transfer buffer (Gloves a must). 5.Cut 4 pieces of Whatman 3MM paper to the same size as the gel and one twice as long to be used as a wick. 6.Set up capillary
Southern Blot Analysis(from Baker lab, university of Florida)
3MM paper to the same size as the gel and one twice as long to be used as a wick.6.Set up capillary blot as per Maniatis.Transfer for at least 6 hours,normally we allow the transfer to proceed overnight.7.Place gel support in Pyrex dish,add transfer
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