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文献和实验7b. Pool the supernatants from step 6 into 500 ml bottles and add DNase-free RNase A and RNase T1 such that the final concentration of RNase A is 40 ug/ml and RNase T1 is 40 U/ml. Incubate in a 37degC water bath for 30 minutes. 8b. Add an equal
and Jaakkola TS. 2003. Comparing the continuous representation of time-series expression profiles to identify differentially expressed genes. Proc Natl Acad Sci U S A, 100(18): 10146-51. 13• Bar-Joseph Z, Gerber GK, Lee TI, Rinaldi NJ, Yoo JY, Robert F
Northern Blotting方法(Howell Lab)
inverted because the RNA pellets on the bottom. (DNA can be recovered from the CsCl layer.) Cut off the tube bottoms (keep inverted) and resuspend the pellet in 100 µl 0.3 M NaOAc (pH 6) buffer. Transfer to a screw-cap 1.5 ml Eppi. Wash tube
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