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文献和实验Measurement of NO and NO Synthase
2 ) or acidified VCl 3 for nitrite/nitrate reduction (see protocol 3 ) NO gas cylinder and stainless steel regulator (100 to 200 ppm NO in N 2 , for preparation of NO gas standards) and N 2 gas
1.5‐mm spacers Peristaltic pump and 0.110‐in. i.d., 2.79‐mm PVC tubing 19‐G needles (8‐in. long; 1.00 × 200–mm) with Luer‐connection (e.g., Rometsch GmbH or Popper & Sons) Gradient maker with two chambers
Marcantonio Lab Protocol Manual
cold room. 3) Aliquot culture into 8 pre-chilled sterile 50 ml polypropylene tubes. Let stand on ice for 10 minutes. 4) Centrifuge cells for 7 minutes at 3000 rpm at 4 degrees. 5) Pour off the supernatant and gently resuspend each pellet in 10 ml
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