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文献和实验CsCl soln. 11) Centrifuge at 40 K for 48 hrs in an SW-55 (or comparable) rotor. Injection DNA Preparation (cont) 12) Pull lower (plasmid) band using a 22.5 gauge needle and a 1 ml syringe. 13) Extract EtBr with water saturated butanol or water
0.4 mls of 10 mgs/ml EtBr. Balance tubes with TE or saturated CsCl soln. 11) Centrifuge at 40 K for 48 hrs in an SW-55 (or comparable) rotor. Injection DNA Preparation (cont) 12) Pull lower (plasmid) band using a 22.5 gauge needle and a 1 ml
phase. On day of injection, spin 1-2 xE6 cells per mouse. Resuspend in IMDM or PBS at 1-2 xE6 cells/0.5ml (do not leave the cells without serum for more than 1 - 2 hr). Transfer cells to syringe using a 16G needle. Inject 0.5ml
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