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文献和实验Coupling Peptides To Resin For Affinity Purification
We use Affigel-10, converting its functional group first to amino and then to iodoacetyl. You can also buy amino resin (it's more expensive) and start at step 6. All washes are performed on a glass-fritted filter
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
, refilling original filter with 450 mL water. Concentrate to 10 mL. Samples can be stored at -20C indefinitely. D. Coupling Add 0.5 mL 1M sodium bicarbonate, pH 9.0 to 50 mM final. Check 1M stock solution periodically for fluctuations
Preparation of Organotypic Hippocampal Slice Cultures Using the Membrane Filter Method
of the membrane filter method ( 3 ). Brain slices are placed on a porous membrane filter that provides a suitable substratum and allows a thin film of media to cover the surface of the slice by capillary action (Fig. 1 ). The membrane inserts are placed
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