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文献和实验Large Scale Tubulin Preparation
here is for a large scale prep (10 cow brains) that yields 1-4 grams of tubulin. The protocol can be scaled down if such a large prep is either not necessary or not doable. For ease of organization, all the pre-prep and day-of-prep activities are listed in outline
Large Scale Tubulin Preparation
Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosphocellulose (PC) column. The procedure described here is for a large scale prep (10 cow brains
followed by removal of copurifying proteins on a phosphocellulose (PC) column. The procedure described here is for a large scale prep (10 cow brains) that yields 1-4 grams of tubulin. The protocol can be scaled down if such a large prep
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