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文献和实验). Purified embryos were resuspended in 100 l of M9 to which 100 l of 1 mg/ml chitinase (Sigma, St. Louis, MO) dissolved in 0.3M mannitol, 50mM HEPES (pH 7.2), 10mM NaCl, 10mM MgCl2 , 2mM DTT was added and shaken vigorously in a microcentrifuge tube for 70
be too dilute, it can easily be precipitated like a water-solubilized sample, and resuspended in a smaller volume with 3M Sodium Acetate and 100% ETOH.For QIAGEN RNeasy (tm) protocols:After eluting the RNA from the columns with water, either dry down the sample
V294.PART II，Chapter 2 Boyden法体外趋化试验
to other stimuli. 2. Materials 1. Standard 48-well chemotaxis chamber (see Fig. 1), including: a lower chamber,a silicone gasket, an upper chamber, and thumb nuts (Neuro Probe; Cabin John,MD). 2. Poretics® polycarbonate: polyvinylpyrrolidone-free
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