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文献和实验1. Run samples out on a gel. For bacterially expressed proteins, generally 5 μl is plenty (1ml cell cμlture; cells resuspeded in 50 μl loading buffer). Run the gels (BioRad mini gels) at 195 V for approximately 40 min. (until samples run close
1. Run samples out on a gel. For bacterially expressed proteins, generally 5 uL is plenty (1ml cell culture; cells resuspeded in 50 ul loading buffer). Run the gels (BioRad mini gels) at 195 V for approximately 40 min. (until samples run
HIGH RESOLUTION GENETIC FOOTPRINTING
9600 thermal cycler with thin-walled 0.2 ml tubes, and AmpliTaq polymerase from Perkin-Elmer. 6.) Materials for preparative agarose gel electrophoresis. We use the Quiaquick gel extraction kit (Qiagen) for all band excision steps, and the Quiaquick
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