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文献和实验重重。 图 1. 利用QIAgenes优化序列,昆虫细胞体外进行膜蛋白表达。 利用 EasyXpress Insect Kit II,crude lysates (T), supernatant (S), and pellet fraction (P) by SDS-PAGE and western blotting using Penta·His antibodies. Note that microsomes with integrated membrane
Protocol for Trichloroacetic Acid (TCA) Precipitation
sperm DNA, Ambion Cat # 9680 ; dilute to 1 mg/ml in water) Glass fiber filters to catch precipitate (e.g., Whatman GF/C filter circle; or Schleicher & Schuell #34 2.7-cm glass filter circles, Cat #10373828) Aqueous scintillation fluid
Analyzing Cancer Samples with SNP Arrays
example, we apply our recently developed and further improved ASCAT (allele-specific copy number analysis of tumors) suite of tools to analyze SNP array data using data from a series of breast carcinomas as an example. We first describe the structure
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