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文献和实验, resuspend each pellet in 40 ml of diatomaceous earth-wash buffer, and centrifuge as above. 11b. Decant the supernatant, resuspend each pellet in 40 ml of acetone, and centrifuge as above. 12b. Decant the supernatant and dry the pellet in a vacuum
Methods for DNA isolation(DNA分离方法)
,and assemble the Prep-A-Gene manifold as described in the manual. 8b.Turn on the vacuum pump and adjust the vacuumlevel to 8 in.Hg,let the membrane dry for 1 minute,and then release the vacuum. 9b.Pour the well mixed samples into the wells of the Prep-A-Gene
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
the plants the day before infiltation). Draw vacuum for 15-20 min for WS and 30 min for Col-0 at a pressure close to 0.05 Bar (we are using an oil pump). 7. Before removing the plants from the vacuum jar place a plastic bag over the pot and beaker. Pull
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