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上海希言科学仪器有限公司
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文献和实验Sample preparation (analytical gels)
phenylmethylsulfonyl fluoridem, 1mM EDTA, and antibiotics: oxacillin 25 μg/ml, gentamycin 50 μg/ml, penicillin 100 U/ml, streptomycin 100 μg/ml, amphotericin B 0.25 μg/ml, nistatin 50 U/ml) to further remove cell debris and blood, and were frozen by immersion in liquid
Sample preparation (analytical gels)
in cold rinse buffer (glutamine-free RPMI 1640 medium containing 5 % fetal calf serum,0.2 mM phenylmethylsulfonyl fluoridem,1mM EDTA,and antibiotics: oxacillin 25 µg/ml,gentamycin 50 µg/ml,penicillin 100 U/ml,streptomycin 100 µg/ml,amphotericin B 0.25 µg
Table of Contents Basic Protocol 1: Reduced‐Scale Large‐Zone Analytical Gel‐Filtration Chromatography Support Protocol 1: Data Analysis
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