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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
15. Spin 10-20 min in microfuge. Wash twice with 70 EtOH and dry pellet. Resuspend in 200 µl of sterile RNase-free gd H2O. Store at -70°C for up to three months. Spectophotometric quantification may be done at this point. 16. Load 1 µl
Marcantonio Lab Protocol Manual
before withdrawing any of the solution. 14) Resuspend the pellet in an additional 20 ul of your low-salt buffer, incubate, spin, and transfer 20 ul of the supernatant into the tube containing the clean DNA. Large-scale Plasmid Prep Large-Scale
A quick RNA mini-prep for Neurospora mycelial cultures
A quick RNA mini-prep for Neurospora mycelial cultures Lindgren, K.M., A. Lichens-Park, J.L. Loros and J.C. Dunlap Dept. of Biochemistry, Dartmouth Medical School, Hanover, NH 03756. Most RNA isolation techniques currently in use
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